GlutaredoxinV1, Main, Exploration, bibRecord, 000133

Production of adeno-associated virus vectors for in vitro and in vivo applications.

Identifieur interne : 000133 ( Main/Exploration ); précédent : 000132; suivant : 000134

Production of adeno-associated virus vectors for in vitro and in vivo applications.

Auteurs : Toyokazu Kimura [États-Unis, Japon] ; Beatriz Ferran [États-Unis] ; Yuko Tsukahara [États-Unis] ; Qifan Shang [États-Unis] ; Suveer Desai [États-Unis] ; Alessandra Fedoce [États-Unis] ; David Richard Pimentel [États-Unis] ; Ivan Luptak [États-Unis] ; Takeshi Adachi [Japon] ; Yasuo Ido [Japon] ; Reiko Matsui [États-Unis] ; Markus Michael Bachschmid [États-Unis]

Source :

Scientific reports [ 2045-2322 ] ; 2019.

RBID : pubmed:31537820

Descripteurs français

KwdFr :
- Animaux (MeSH), Cellules HEK293 (MeSH), Charge virale (MeSH), Dependovirus (croissance et développement), Dependovirus (génétique), Dependovirus (isolement et purification), Foie (métabolisme), Glutarédoxines (antagonistes et inhibiteurs), Glutarédoxines (métabolisme), Humains (MeSH), Lignée cellulaire (MeSH), Muscles squelettiques (métabolisme), Mâle (MeSH), Petit ARN interférent (génétique), Polyéthylènes (composition chimique), Précipitation chimique (MeSH), Régulation négative (MeSH), Transduction génétique (MeSH), Vecteurs génétiques (génétique), Étude de validation de principe (MeSH).
MESH :
- antagonistes et inhibiteurs : Glutarédoxines.
- composition chimique : Polyéthylènes.
- croissance et développement : Dependovirus.
- génétique : Dependovirus, Petit ARN interférent, Vecteurs génétiques.
- isolement et purification : Dependovirus.
- métabolisme : Foie, Glutarédoxines, Muscles squelettiques.
- Animaux, Cellules HEK293, Charge virale, Humains, Lignée cellulaire, Mâle, Précipitation chimique, Régulation négative, Transduction génétique, Étude de validation de principe.

English descriptors

KwdEn :
- Animals (MeSH), Cell Line (MeSH), Chemical Precipitation (MeSH), Dependovirus (genetics), Dependovirus (growth & development), Dependovirus (isolation & purification), Down-Regulation (MeSH), Genetic Vectors (genetics), Glutaredoxins (antagonists & inhibitors), Glutaredoxins (metabolism), HEK293 Cells (MeSH), Humans (MeSH), Liver (metabolism), Male (MeSH), Muscle, Skeletal (metabolism), Polyethylenes (chemistry), Proof of Concept Study (MeSH), RNA, Small Interfering (genetics), Transduction, Genetic (MeSH), Viral Load (MeSH).
MESH :
- chemical , antagonists & inhibitors : Glutaredoxins.
- chemical , chemistry : Polyethylenes.
- genetics : Dependovirus, Genetic Vectors, RNA, Small Interfering.
- growth & development : Dependovirus.
- isolation & purification : Dependovirus.
- chemical , metabolism : Glutaredoxins, Liver, Muscle, Skeletal.
- Animals, Cell Line, Chemical Precipitation, Down-Regulation, HEK293 Cells, Humans, Male, Proof of Concept Study, Transduction, Genetic, Viral Load.

Abstract

Delivering and expressing a gene of interest in cells or living animals has become a pivotal technique in biomedical research and gene therapy. Among viral delivery systems, adeno-associated viruses (AAVs) are relatively safe and demonstrate high gene transfer efficiency, low immunogenicity, stable long-term expression, and selective tissue tropism. Combined with modern gene technologies, such as cell-specific promoters, the Cre/lox system, and genome editing, AAVs represent a practical, rapid, and economical alternative to conditional knockout and transgenic mouse models. However, major obstacles remain for widespread AAV utilization, such as impractical purification strategies and low viral quantities. Here, we report an improved protocol to produce serotype-independent purified AAVs economically. Using a helper-free AAV system, we purified AAVs from HEK293T cell lysates and medium by polyethylene glycol precipitation with subsequent aqueous two-phase partitioning. Furthermore, we then implemented an iodixanol gradient purification, which resulted in preparations with purities adequate for in vivo use. Of note, we achieved titers of 10¹⁰-10¹¹ viral genome copies per µl with a typical production volume of up to 1 ml while requiring five times less than the usual number of HEK293T cells used in standard protocols. For proof of concept, we verified in vivo transduction via Western blot, qPCR, luminescence, and immunohistochemistry. AAVs coding for glutaredoxin-1 (Glrx) shRNA successfully inhibited Glrx expression by ~66% in the liver and skeletal muscle. Our study provides an improved protocol for a more economical and efficient purified AAV preparation.

DOI: 10.1038/s41598-019-49624-w
PubMed: 31537820
PubMed Central: PMC6753157

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000111
to stream Main, to step Curation: 000111

Le document en format XML

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<author><name sortKey="Fedoce, Alessandra" sort="Fedoce, Alessandra" uniqKey="Fedoce A" first="Alessandra" last="Fedoce">Alessandra Fedoce</name>
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<author><name sortKey="Luptak, Ivan" sort="Luptak, Ivan" uniqKey="Luptak I" first="Ivan" last="Luptak">Ivan Luptak</name>
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<author><name sortKey="Ido, Yasuo" sort="Ido, Yasuo" uniqKey="Ido Y" first="Yasuo" last="Ido">Yasuo Ido</name>
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<author><name sortKey="Matsui, Reiko" sort="Matsui, Reiko" uniqKey="Matsui R" first="Reiko" last="Matsui">Reiko Matsui</name>
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<country xml:lang="fr">États-Unis</country>
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<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bachschmid, Markus Michael" sort="Bachschmid, Markus Michael" uniqKey="Bachschmid M" first="Markus Michael" last="Bachschmid">Markus Michael Bachschmid</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Production of adeno-associated virus vectors for in vitro and in vivo applications.</title>
<author><name sortKey="Kimura, Toyokazu" sort="Kimura, Toyokazu" uniqKey="Kimura T" first="Toyokazu" last="Kimura">Toyokazu Kimura</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Cardiovascular Medicine, National Defense Medical College, Tokorozawa</wicri:regionArea>
<wicri:noRegion>Tokorozawa</wicri:noRegion>
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<author><name sortKey="Ferran, Beatriz" sort="Ferran, Beatriz" uniqKey="Ferran B" first="Beatriz" last="Ferran">Beatriz Ferran</name>
<affiliation wicri:level="3"><nlm:affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsukahara, Yuko" sort="Tsukahara, Yuko" uniqKey="Tsukahara Y" first="Yuko" last="Tsukahara">Yuko Tsukahara</name>
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<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
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<author><name sortKey="Shang, Qifan" sort="Shang, Qifan" uniqKey="Shang Q" first="Qifan" last="Shang">Qifan Shang</name>
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<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
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<region type="state">Massachusetts</region>
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</affiliation>
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<author><name sortKey="Desai, Suveer" sort="Desai, Suveer" uniqKey="Desai S" first="Suveer" last="Desai">Suveer Desai</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fedoce, Alessandra" sort="Fedoce, Alessandra" uniqKey="Fedoce A" first="Alessandra" last="Fedoce">Alessandra Fedoce</name>
<affiliation wicri:level="3"><nlm:affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pimentel, David Richard" sort="Pimentel, David Richard" uniqKey="Pimentel D" first="David Richard" last="Pimentel">David Richard Pimentel</name>
<affiliation wicri:level="3"><nlm:affiliation>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Luptak, Ivan" sort="Luptak, Ivan" uniqKey="Luptak I" first="Ivan" last="Luptak">Ivan Luptak</name>
<affiliation wicri:level="3"><nlm:affiliation>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Adachi, Takeshi" sort="Adachi, Takeshi" uniqKey="Adachi T" first="Takeshi" last="Adachi">Takeshi Adachi</name>
<affiliation wicri:level="1"><nlm:affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Cardiovascular Medicine, National Defense Medical College, Tokorozawa</wicri:regionArea>
<wicri:noRegion>Tokorozawa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ido, Yasuo" sort="Ido, Yasuo" uniqKey="Ido Y" first="Yasuo" last="Ido">Yasuo Ido</name>
<affiliation wicri:level="1"><nlm:affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Cardiovascular Medicine, National Defense Medical College, Tokorozawa</wicri:regionArea>
<wicri:noRegion>Tokorozawa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Matsui, Reiko" sort="Matsui, Reiko" uniqKey="Matsui R" first="Reiko" last="Matsui">Reiko Matsui</name>
<affiliation wicri:level="3"><nlm:affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA. rmatsui@bu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bachschmid, Markus Michael" sort="Bachschmid, Markus Michael" uniqKey="Bachschmid M" first="Markus Michael" last="Bachschmid">Markus Michael Bachschmid</name>
<affiliation wicri:level="3"><nlm:affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA. bach@bu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston</wicri:regionArea>
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<region type="state">Massachusetts</region>
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</affiliation>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Cell Line (MeSH)</term>
<term>Chemical Precipitation (MeSH)</term>
<term>Dependovirus (genetics)</term>
<term>Dependovirus (growth & development)</term>
<term>Dependovirus (isolation & purification)</term>
<term>Down-Regulation (MeSH)</term>
<term>Genetic Vectors (genetics)</term>
<term>Glutaredoxins (antagonists & inhibitors)</term>
<term>Glutaredoxins (metabolism)</term>
<term>HEK293 Cells (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Liver (metabolism)</term>
<term>Male (MeSH)</term>
<term>Muscle, Skeletal (metabolism)</term>
<term>Polyethylenes (chemistry)</term>
<term>Proof of Concept Study (MeSH)</term>
<term>RNA, Small Interfering (genetics)</term>
<term>Transduction, Genetic (MeSH)</term>
<term>Viral Load (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Cellules HEK293 (MeSH)</term>
<term>Charge virale (MeSH)</term>
<term>Dependovirus (croissance et développement)</term>
<term>Dependovirus (génétique)</term>
<term>Dependovirus (isolement et purification)</term>
<term>Foie (métabolisme)</term>
<term>Glutarédoxines (antagonistes et inhibiteurs)</term>
<term>Glutarédoxines (métabolisme)</term>
<term>Humains (MeSH)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Muscles squelettiques (métabolisme)</term>
<term>Mâle (MeSH)</term>
<term>Petit ARN interférent (génétique)</term>
<term>Polyéthylènes (composition chimique)</term>
<term>Précipitation chimique (MeSH)</term>
<term>Régulation négative (MeSH)</term>
<term>Transduction génétique (MeSH)</term>
<term>Vecteurs génétiques (génétique)</term>
<term>Étude de validation de principe (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Glutaredoxins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Polyethylenes</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Glutarédoxines</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Polyéthylènes</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Dependovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Dependovirus</term>
<term>Genetic Vectors</term>
<term>RNA, Small Interfering</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Dependovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dependovirus</term>
<term>Petit ARN interférent</term>
<term>Vecteurs génétiques</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Dependovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Dependovirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutaredoxins</term>
<term>Liver</term>
<term>Muscle, Skeletal</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Foie</term>
<term>Glutarédoxines</term>
<term>Muscles squelettiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chemical Precipitation</term>
<term>Down-Regulation</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>Male</term>
<term>Proof of Concept Study</term>
<term>Transduction, Genetic</term>
<term>Viral Load</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules HEK293</term>
<term>Charge virale</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Mâle</term>
<term>Précipitation chimique</term>
<term>Régulation négative</term>
<term>Transduction génétique</term>
<term>Étude de validation de principe</term>
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<front><div type="abstract" xml:lang="en">Delivering and expressing a gene of interest in cells or living animals has become a pivotal technique in biomedical research and gene therapy. Among viral delivery systems, adeno-associated viruses (AAVs) are relatively safe and demonstrate high gene transfer efficiency, low immunogenicity, stable long-term expression, and selective tissue tropism. Combined with modern gene technologies, such as cell-specific promoters, the Cre/lox system, and genome editing, AAVs represent a practical, rapid, and economical alternative to conditional knockout and transgenic mouse models. However, major obstacles remain for widespread AAV utilization, such as impractical purification strategies and low viral quantities. Here, we report an improved protocol to produce serotype-independent purified AAVs economically. Using a helper-free AAV system, we purified AAVs from HEK293T cell lysates and medium by polyethylene glycol precipitation with subsequent aqueous two-phase partitioning. Furthermore, we then implemented an iodixanol gradient purification, which resulted in preparations with purities adequate for in vivo use. Of note, we achieved titers of 10<sup>10</sup>
-10<sup>11</sup>
 viral genome copies per µl with a typical production volume of up to 1 ml while requiring five times less than the usual number of HEK293T cells used in standard protocols. For proof of concept, we verified in vivo transduction via Western blot, qPCR, luminescence, and immunohistochemistry. AAVs coding for glutaredoxin-1 (Glrx) shRNA successfully inhibited Glrx expression by ~66% in the liver and skeletal muscle. Our study provides an improved protocol for a more economical and efficient purified AAV preparation.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31537820</PMID>
<DateCompleted><Year>2020</Year>
<Month>10</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>26</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>9</Volume>
<Issue>1</Issue>
<PubDate><Year>2019</Year>
<Month>09</Month>
<Day>19</Day>
</PubDate>
</JournalIssue>
<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Production of adeno-associated virus vectors for in vitro and in vivo applications.</ArticleTitle>
<Pagination><MedlinePgn>13601</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-49624-w</ELocationID>
<Abstract><AbstractText>Delivering and expressing a gene of interest in cells or living animals has become a pivotal technique in biomedical research and gene therapy. Among viral delivery systems, adeno-associated viruses (AAVs) are relatively safe and demonstrate high gene transfer efficiency, low immunogenicity, stable long-term expression, and selective tissue tropism. Combined with modern gene technologies, such as cell-specific promoters, the Cre/lox system, and genome editing, AAVs represent a practical, rapid, and economical alternative to conditional knockout and transgenic mouse models. However, major obstacles remain for widespread AAV utilization, such as impractical purification strategies and low viral quantities. Here, we report an improved protocol to produce serotype-independent purified AAVs economically. Using a helper-free AAV system, we purified AAVs from HEK293T cell lysates and medium by polyethylene glycol precipitation with subsequent aqueous two-phase partitioning. Furthermore, we then implemented an iodixanol gradient purification, which resulted in preparations with purities adequate for in vivo use. Of note, we achieved titers of 10<sup>10</sup>
-10<sup>11</sup>
 viral genome copies per µl with a typical production volume of up to 1 ml while requiring five times less than the usual number of HEK293T cells used in standard protocols. For proof of concept, we verified in vivo transduction via Western blot, qPCR, luminescence, and immunohistochemistry. AAVs coding for glutaredoxin-1 (Glrx) shRNA successfully inhibited Glrx expression by ~66% in the liver and skeletal muscle. Our study provides an improved protocol for a more economical and efficient purified AAV preparation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kimura</LastName>
<ForeName>Toyokazu</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ferran</LastName>
<ForeName>Beatriz</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tsukahara</LastName>
<ForeName>Yuko</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shang</LastName>
<ForeName>Qifan</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Desai</LastName>
<ForeName>Suveer</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fedoce</LastName>
<ForeName>Alessandra</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pimentel</LastName>
<ForeName>David Richard</ForeName>
<Initials>DR</Initials>
<AffiliationInfo><Affiliation>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Luptak</LastName>
<ForeName>Ivan</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>Cardiology, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Adachi</LastName>
<ForeName>Takeshi</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ido</LastName>
<ForeName>Yasuo</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Cardiovascular Medicine, National Defense Medical College, Tokorozawa, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Matsui</LastName>
<ForeName>Reiko</ForeName>
<Initials>R</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-8132-2823</Identifier>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA. rmatsui@bu.edu.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bachschmid</LastName>
<ForeName>Markus Michael</ForeName>
<Initials>MM</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-0748-5528</Identifier>
<AffiliationInfo><Affiliation>Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, USA. bach@bu.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>T32 HL007224</GrantID>
<Acronym>HL</Acronym>
<Agency>NHLBI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R01 HL133013</GrantID>
<Acronym>HL</Acronym>
<Agency>NHLBI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R03 AG051857</GrantID>
<Acronym>AG</Acronym>
<Agency>NIA NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R01 DK103750</GrantID>
<Acronym>DK</Acronym>
<Agency>NIDDK NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>UL1 TR001430</GrantID>
<Acronym>TR</Acronym>
<Agency>NCATS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>09</Month>
<Day>19</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Sci Rep</MedlineTA>
<NlmUniqueID>101563288</NlmUniqueID>
<ISSNLinking>2045-2322</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C516006">Glrx protein, mouse</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011095">Polyethylenes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C045578">polyethylene chloride</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011232" MajorTopicYN="N">Chemical Precipitation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000229" MajorTopicYN="N">Dependovirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName>
<QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008099" MajorTopicYN="N">Liver</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018482" MajorTopicYN="N">Muscle, Skeletal</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011095" MajorTopicYN="N">Polyethylenes</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000075082" MajorTopicYN="N">Proof of Concept Study</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014161" MajorTopicYN="N">Transduction, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019562" MajorTopicYN="N">Viral Load</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>09</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>07</Month>
<Day>11</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>9</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>9</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>10</Month>
<Day>27</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31537820</ArticleId>
<ArticleId IdType="doi">10.1038/s41598-019-49624-w</ArticleId>
<ArticleId IdType="pii">10.1038/s41598-019-49624-w</ArticleId>
<ArticleId IdType="pmc">PMC6753157</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Japon</li>
<li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
</region>
<settlement><li>Boston</li>
</settlement>
</list>
<tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Kimura, Toyokazu" sort="Kimura, Toyokazu" uniqKey="Kimura T" first="Toyokazu" last="Kimura">Toyokazu Kimura</name>
</region>
<name sortKey="Bachschmid, Markus Michael" sort="Bachschmid, Markus Michael" uniqKey="Bachschmid M" first="Markus Michael" last="Bachschmid">Markus Michael Bachschmid</name>
<name sortKey="Desai, Suveer" sort="Desai, Suveer" uniqKey="Desai S" first="Suveer" last="Desai">Suveer Desai</name>
<name sortKey="Fedoce, Alessandra" sort="Fedoce, Alessandra" uniqKey="Fedoce A" first="Alessandra" last="Fedoce">Alessandra Fedoce</name>
<name sortKey="Ferran, Beatriz" sort="Ferran, Beatriz" uniqKey="Ferran B" first="Beatriz" last="Ferran">Beatriz Ferran</name>
<name sortKey="Luptak, Ivan" sort="Luptak, Ivan" uniqKey="Luptak I" first="Ivan" last="Luptak">Ivan Luptak</name>
<name sortKey="Matsui, Reiko" sort="Matsui, Reiko" uniqKey="Matsui R" first="Reiko" last="Matsui">Reiko Matsui</name>
<name sortKey="Pimentel, David Richard" sort="Pimentel, David Richard" uniqKey="Pimentel D" first="David Richard" last="Pimentel">David Richard Pimentel</name>
<name sortKey="Shang, Qifan" sort="Shang, Qifan" uniqKey="Shang Q" first="Qifan" last="Shang">Qifan Shang</name>
<name sortKey="Tsukahara, Yuko" sort="Tsukahara, Yuko" uniqKey="Tsukahara Y" first="Yuko" last="Tsukahara">Yuko Tsukahara</name>
</country>
<country name="Japon"><noRegion><name sortKey="Kimura, Toyokazu" sort="Kimura, Toyokazu" uniqKey="Kimura T" first="Toyokazu" last="Kimura">Toyokazu Kimura</name>
</noRegion>
<name sortKey="Adachi, Takeshi" sort="Adachi, Takeshi" uniqKey="Adachi T" first="Takeshi" last="Adachi">Takeshi Adachi</name>
<name sortKey="Ido, Yasuo" sort="Ido, Yasuo" uniqKey="Ido Y" first="Yasuo" last="Ido">Yasuo Ido</name>
</country>
</tree>
</affiliations>
</record>

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Production of adeno-associated virus vectors for in vitro and in vivo applications.

Production of adeno-associated virus vectors for in vitro and in vivo applications.

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